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human β ngf  (R&D Systems)


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    R&D Systems human β ngf
    Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B2+ngf/Recombinant+Human+beta-NGF+Protein/pm41999764-275-45-47
    Average 95 stars, based on 111 article reviews
    human β ngf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
    Article Snippet: .. The DRG bulb was then cut into 3 mm sections and placed in 5 mL of pre-warmed digestion enzyme containing 1 mg/mL of Stemzyme I (Worthington Biochemical, LS004106 ), 0.1 mg/mL of DNAse I (Worthington Biochemical, LS002139 ), and 10 ng/mL of recombinant human β-NGF (R&D Systems, 256-GF) in HBSS without calcium and magnesium (Thermo Scientific, 14170-112). ..

    Article Title: Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy.
    Article Snippet: .. TheDRGbulbwas then cut into 3mmsections andplaced in 5mL of pre-warmed digestion enzyme containing 1mg/mL of Stemzyme I (Worthington Biochemical, LS004106), 0.1mg/mL of DNAse I (Worthington Biochemical, LS002139), and 10 ng/mL of recombinant human β-NGF (R&D Systems, 256-GF) in HBSS without calcium and magnesium (Thermo Scientific, 14170-112). ..

    Article Title: Enabling wider access to human molecular neuroscience research in pain: A simple preservation method for human dorsal root ganglion neurons in Hibernate A media
    Article Snippet: .. The bulb of each DRG was diced into 3 mm x 3 mm sections and placed in 5 mL of prewarmed (37 °C) digestion enzyme consisting of 1 mg/mL of Stemxyme I (Worthington Biochemical, LS004106), 0.1 mg/mL of DNAse I (Worthington Biochemical, LS002139), and 10 ng/mL of recombinant human β-NGF (R&D Systems, 256-GF) in HBSS without calcium and magnesium (Thermo Scientific, 14170-112). ..

    Article Title: An improved preservation method for human dorsal root ganglion neurons enables wider access to human molecular pain neuroscience.
    Article Snippet: .. The bulb of each DRG was diced into 3 mm × 3 mm sections and placed in 5 mL of prewarmed (37◦C) digestion enzyme consisting of 1 mg/mL of Stemxyme I (Worthington Biochemical), 0.1 mg/mL of DNAse I (Worthington Biochemical), and 10 ng/mL of recombinant human β-NGF (R&D Systems) in HBSS without calcium and magnesium (Thermo Scientific). ..

    Positive Control:

    Article Title: Synovial fibroblast gene expression is associated with sensory nerve growth and pain in rheumatoid arthritis
    Article Snippet: The cell pellet was resuspended in 1× Neurobasal Plus medium (Thermo Fisher Scientific, A3582901) containing B27 (Thermo Fisher Scientific, 17504001) diluted 1:50, GlutaMAX (Thermo Fisher Scientific, 35050061) diluted 1:100, and gentamicin sulfate (Abbott Laboratories) and then plated onto the precoated slides. .. Human β-NGF (0.1 μg/ml; R&D Systems, 256-GF) was added to the medium of the positive control slide chamber. .. A total of 500,000 synovial fibroblasts from low inflammatory RA, between five and eight passages, were plated in each well of six-well cell culture plates and covered with 2 ml of Neurobasal medium (Thermo Fisher Scientific, 21103049) with added 2% B-27 supplement (Thermo Fisher Scientific, 17504044), 1% GlutaMAX supplement (Thermo Fisher Scientific, 35050061), and 0.04% gentamicin sulfate.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Plasma Nerve Growth Factor Is Associated With Early Postoperative Shoulder Stiffness in Patients With Degenerative Rotator Cuff Tears.
    Article Snippet: .. Human β- NGF (pg/mL) was evaluated using the DuoSet ELISA system (R&D Systems, USA). ..

    Article Title: Plasma Nerve Growth Factor Is Associated With Early Postoperative Shoulder Stiffness in Patients With Degenerative Rotator Cuff Tears
    Article Snippet: .. Human β‐NGF (pg/mL) was evaluated using the DuoSet ELISA system (R&D Systems, USA). ..



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    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced <t>by</t> <t>stimulation</t> with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , <t>NGF</t> , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.
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    recombinant human β ngf protein  (R&D Systems)
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    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced <t>by</t> <t>stimulation</t> with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , <t>NGF</t> , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.
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    Image Search Results


    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis

    doi: 10.1038/s41467-026-70000-6

    Figure Lengend Snippet: Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Article Snippet: The mixture was placed in an incubator at 37 °C with 5% CO 2 for 30 min, followed by stimulation with recombinant human NGF (R&D Systems, 256-GF) at a final concentration 0.1 μg/mL (or PBS as negative control) and the resulting mixtures were further incubated for 20 min. Next, anti-CD123-APC (Miltenyi Biotec, 130-115-265) and anti-CD203c-PE (Miltenyi Biotec, 130-092-343) antibodies were added and continued to incubate for another 15 minutes.

    Techniques: RNA Expression, Control, Expressing, RNA Sequencing